Catalog Number - 68100-05P-2

The 68100‑05P‑2 Cogent Method Development Kit provides a complete multi‑selectivity UHPLC platform, offering five complementary reversed‑phase chemistries for rapid method development and optimization.

Each column in the kit is packed with 1.8 µm particles on high‑purity silica with 100 Å pore size, delivering high efficiency, excellent peak shape, and reproducible performance across a wide range of analytes. The narrow‑bore 2.1 mm format is ideal for LC‑MS and low‑flow UHPLC applications, maximizing sensitivity while minimizing solvent consumption.

This kit enables quick screening of retention mechanisms, allowing selection of the most suitable stationary phase for complex separations without the need to purchase columns individually.

Included Column Chemistries

This kit includes five complementary RP phases designed to explore different selectivity profiles:

• Cogent RP™ C8 – Moderate hydrophobic retention

• Cogent RP™ C18 – Strong hydrophobic retention (general purpose)

• Cogent RP™ Cyano – Polar selectivity with reversed‑phase behavior

• Cogent RP™ Phenyl – π‑π interactions for aromatic compounds

• Cogent RP™ PFP – Unique selectivity for polar and halogenated compounds

👉 Each chemistry provides a different interaction mechanism, enabling efficient method scouting and optimization.

50 mm Length – Fast Method Screening

All columns are 50 mm in length, optimized for rapid screening and method evaluation.

• Short run times for high throughput

• Efficient comparison of selectivity across phases

• Reduced solvent consumption during development

✅ Ideal for fast decision-making in method development workflows

Flow Rate & System Requirements (2.1 mm ID, 1.8 µm)

⚠️ Requires UHPLC system capability due to high backpressure

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.25–0.35 mL/min

• Requires precise low‑flow pump control

• Compatible with low‑volume LC‑MS or UHPLC detectors

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Use a Method Development Kit

Rapid Selectivity Screening

• Evaluate multiple stationary phases quickly

• Identify optimal chemistry for difficult separations

Reduce Method Development Time

• Eliminates trial‑and‑error ordering of individual columns

• Speeds up method optimization

Comprehensive Coverage

• Covers hydrophobic, polar, aromatic, and halogen interactions

• Enables broad applicability across compound classes

Cogent RP™ UHPLC Advantages

High Efficiency (1.8 µm Particles)

• High plate counts and peak capacity

• Faster separations with maintained resolution

High Purity Silica

• Consistent column‑to‑column performance

• Improved reproducibility and reliability

Low‑Flow Compatibility (2.1 mm ID)

• Reduced solvent consumption

• Enhanced LC‑MS sensitivity

Typical Applications

• UHPLC method development

• LC‑MS method optimization

• Pharmaceutical method screening

• Complex mixture analysis

• New method creation and validation

Important Usage Notes

• ⚠️ Requires UHPLC system (high‑pressure capability)

• Designed for low‑flow LC‑MS and UHPLC workflows

• Ideal for method development and screening applications